文獻(xiàn):Gutenberg-style printing of self-assembled metal nanoparticle Aarays: electrostatic nanoparticle immobilisation and DNA-mediated transfer

作者： Zheng, Yuanhui

文獻(xiàn)鏈接：https://bridges.monash.edu/articles/thesis/Gutenberg-style_printing_of_self-assembled_metal_nanoparticle_Aarays_electrostatic_nanoparticle_immobilisation_and_DNA-mediated_transfer/4621498

摘要：

 HS–PEG–COOH was diluted in citrate solution while stirring at room temperature. An amount of DTT was added in the solution with molar ratio DTT/HS–PEG–COOH about 1/1. Here the DTT produces the free sulfhydryl bonds. The solution DTT–HS–PEG–COOH then was added into primary AuNPs solution while stirring at room temperature for about 15?min. The minimum amount of HS–PEG–COOH required to stabilize AuNPs was determined by the method similar to the BSA case. Two serial dilutions of the HS–PEG–COOH–AuNPs (Au@PEG) at pH=4 and pH=6.5 were prepared, varying the quantities of HS–PEG–COOH from 0 to 150?μg in 1?ml of AuNPs solution (μg? ml ?1), and followed by UV-VIS spectrophotometric analysis. The minimum amount of HS–PEG–COOH necessary to coat AuNPs was deduced graphically from the concentration with which the absorbance at pH=4 becomes nearly constant. All of these solutions were measured by UV-Vis spectrophotometry. The size and shape of AuNPs were characterized by a transmission electron microscope (TEM).

在室溫下攪拌的同時，將HS-PEG-COOH稀釋在檸檬酸鹽溶液中。在溶液中加入一定量的DTT，DTT/HS–PEG–COOH的摩爾比約為1/1。在這里，DTT產(chǎn)生游離巰基鍵。然后將DTT-HS-PEG-COOH溶液加入到初級AuNP溶液中，同時在室溫下攪拌約15分鐘?min。穩(wěn)定AuNP所需的HS-PEG-COOH的最小量是通過類似于BSA的方法確定的。

HS-PEG-COOH-AuNPs的兩種系列稀釋液(Au@PEG)在pH=4和pH=6.5的條件下，將HS-PEG-COOH的量從0變化到150?μg/1?ml金納米顆粒溶液（μg? ml-1），然后進(jìn)行UV-VIS分光光度分析。根據(jù)pH=4時吸光度幾乎恒定的濃度，以圖形方式推導(dǎo)出涂覆AuNP所需的最小HS-PEG-COOH量。所有這些溶液都通過紫外-可見分光光度法進(jìn)行了測量。通過透射電子顯微鏡（TEM）對金納米顆粒的尺寸和形狀進(jìn)行了表征。

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