文獻(xiàn)：Surface Functionalized Gold Nanorods: Tracking and Observing Live Cell via Three Optical Signals

作者：Qiu, Xiaozhong; Wang, Yaling; Cui, Yanyan; Sun, Zhipeng; Liu, Ru; Huang, Wenhua; Zhao, Feng; Zu, Yan; Yuan, Hui; Chang, Xueling; Gao, and Xueyun

文獻(xiàn)鏈接：https://www.ingentaconnect.com/contentone/asp/jnn/2012/00000012/00000009/art00004

摘要：

Surface functionalized gold nanorods (GNRs) with three optical signals and the ability to track and observe live cells was investigated. The Cetyltrimethyl Ammonium Bromide (CTAB) was used as the shape-directing agent to develop GNRs with uniform size and aspect ratio. The as-synthesized GNRs can strongly enhance the Raman signal of the attached MGITC on the GNRs. Polyethylene glycol (SH-PEG-COOH) and SH-PEG-FITC were attached with GNRs through ligand exchange with CTAB. They can stabilize the GNRs, reduce the cytotoxicity and provide fluorescence signal. A cell-penetrating peptide was covalently conjugated with SH-PEG-COOH to assist the GNRs to enter living cell. After cultured with the MCF-7 cells, the surfaced functionalized Au nanorods can enter the MCF-7 cells and be tracking with enhanced MGITC Raman signal, FITC fluorescence, and Rayleigh scatter signal.

研究了具有三種光信號(hào)的表面功能化金納米棒（GNR）以及跟蹤和觀察活細(xì)胞的能力。使用十六烷基三甲基溴化銨（CTAB）作為形狀導(dǎo)向劑，開發(fā)出尺寸和縱橫比均勻的GNR。合成的GNR可以強(qiáng)烈增強(qiáng)GNR上附著的MGITC的拉曼信號(hào)。

聚乙二醇（SH-PEG-COOH）和SH-PEG-FITC通過(guò)與CTAB的配體交換與GNR連接。它們可以穩(wěn)定GNR，降低細(xì)胞毒性并提供熒光信號(hào)。細(xì)胞穿透肽與SH-PEG-COOH共價(jià)結(jié)合，以幫助GNR進(jìn)入活細(xì)胞。與MCF-7細(xì)胞一起培養(yǎng)后，表面官能化的Au納米棒可以進(jìn)入MCF-7細(xì)胞，并用增強(qiáng)的MGITC拉曼信號(hào)、FITC熒光和瑞利散射信號(hào)進(jìn)行跟蹤。

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