文獻(xiàn)：Preparation and Characterization of Folate-Targeted Fe3O4 Nanoparticle Codelivering Cisplatin and TFPI-2 Plasmid DNA for Nasopharyngeal Carcinoma Therapy

作者：Juan Zhang, Huanhuan Weng, Xiangwan Miao, Quanming Li, Siqi Wang, Huifen Xie, Tao Liu, Minqiang Xie

文獻(xiàn)鏈接：https://onlinelibrary.wiley.com/doi/full/10.1155/2017/2849801

摘要：

1H NMR was determined to confirm the synthesis of FA-PEG-PEI (the spectrum and relevant chemical structural formula were shown in Figure 1). The characteristic peaks from 2.0 to 3.0?ppm (k, j) were attributed to -CH2CH2NH- group of PEI, the peaks at 3.66?ppm (h) were referred to -CH2CH2O- group of PEG, and the peaks from 6.5 to 9.0?ppm (a, b, c) were related to the H protons of benzene ring in FA [25]. The data suggested a successful combination of FA-PEG-PEI.

The possible functional groups responsible for the synthesis of FA-PEG-PEI were further confirmed by FT-IR spectroscopy. Meanwhile, the FT-IR spectra of FA, NH2-PEG-OH, and NH2-PEG-COOH were also examined. The terminal hydroxyl groups of NH2-PEG-OH were of carboxylation and then combined with the amino groups of PEI via amidation reaction. As shown in Figure 2, the FT-IR spectra of terminal hydroxyl groups of (b) NH2-PEG-OH showed the presence of stretching vibration peak of -OH at 3440?cm?1, which shifted to a new stretching vibration peak of -C=O at 1629?cm?1 in (c) NH2-PEG-COOH after carboxylation. The FT-IR spectra of (d) FA-PEG-PEI displayed a broad peak at 3350–3250?cm?1, which refers to -NH2 and -NH- groups of PEI. The peaks at 1606–1460?cm?1 correspond to the benzene ring of FA. In addition, the peak at 1110?cm?1 is attributed to the symmetrical stretching vibration of -C-O-C- group in PEG, which declares the conjugation of FA-PEG-PEI.

測(cè)定1H NMR以確認(rèn)FA-PEG-PEI的合成。特征峰值在2.0到3.0之間?ppm（k，j）歸因于PEI的-CH2CH2NH-基團(tuán)，峰值在3.66?ppm（h）是指PEG的-CH2CH2O-基團(tuán)，峰在6.5至9.0之間?ppm（a，b，c）與FA中苯環(huán)的H質(zhì)子有關(guān)。數(shù)據(jù)表明，F(xiàn)A-PEG-PEI的組合是成功的。

FT-IR光譜進(jìn)一步證實(shí)了可能負(fù)責(zé)FA-PEG-PEI合成的官能團(tuán)。同時(shí)，還研究了FA、NH2-PEG-OH和NH2-PEG-COOH的傅里葉變換紅外光譜。

NH2-PEG-OH的末端羥基被羧化，然后通過(guò)酰胺化反應(yīng)與PEI的氨基結(jié)合。如圖2所示，（b）NH2-PEG-OH末端羥基的FT-IR光譜顯示，在3440處存在-OH的伸縮振動(dòng)峰?cm-1，在1629處移動(dòng)到新的拉伸振動(dòng)峰-C=O?羧化后，在（c）NH2-PEG-COOH中加入cm-1。（d）FA-PEG-PEI的FT-IR光譜在3350-3250處顯示了一個(gè)寬峰?cm-1表示PEI的-NH2和-NH-基團(tuán)。1606年至1460年的峰值?cm-1對(duì)應(yīng)于FA的苯環(huán)。此外，1110處的峰?cm-1歸因于PEG中-C-O-C-基團(tuán)的對(duì)稱(chēng)伸縮振動(dòng)，這宣告了FA-PEG-PEI的共軛。

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