文獻：Au Nanocage Functionalized with Ultra-small Fe3O4 Nanoparticles for Targeting T1–T2Dual MRI and CT Imaging of Tumor

作者:Guannan Wang, Wei Gao, Xuanjun Zhang & Xifan Mei 

文獻鏈接：https://www.nature.com/articles/srep28258

摘要：

The targeting ability of F-AuNC@Fe3O4 to folate receptor-overexpressed cancer cells was studied using A549 cells as an example. To more directly display the targeting capabilities of the F-AuNC@Fe3O4, the fluorescence Cy5-PEG-SH was selected to label the nanoparticls. In order to give clear fluorescence imaging and retain the ability of bio-functionalization, firstly, the mixture of COOH-PEG-SH and fluorescence Cy5-PEG-SH with 9:1 ratio was used to functionalize the surface of AuNC@Fe3O4 and then fluorescent AuNC@Fe3O4 was conjugated with targeting molecular folic acid to form the fluorescent F-AuNC@Fe3O4 for targeting cancer cell. As a control group, the fluorescent AuNC@Fe3O4 without surface folic acid also was used, which showed similar physical properties to those of fluorescent F-AuNC@Fe3O4. Figure 4a,b row show the confocal images of A549 cells after incubation with fluorescent F-AuNC@Fe3O4 and AuNC@Fe3O4 suspensions at a concentration of 0.5?mg/mL for one hour, respectively. The fluorescence image shown in Fig. 4a(i) clearly shows the successful internalization of the fluorescent F-AuNC@Fe3O4 into A549 cells. The much brighter red emission observed in Fig. 4a(i) as compared to that in Fig. 4b(i) (A549 cell incubation with fluorescent AuNC@Fe3O4) indicates that more F-AuNC@Fe3O4 can be internalized into A549 cells via folate receptor-mediated endocytosis and the F-AuNC@Fe3O4 have more capability for cancer targeting recognition. These results confirm the F-AuNC@Fe3O4 can be effectively targeted to the folate receptor expressed cancer cells.

為了更直接地展示F-AuNC@Fe3O4，選擇熒光Cy5-PEG-SH標記納米顆粒。為了獲得清晰的熒光成像并保持生物功能化的能力，首先，使用比例為9:1的COOH-PEG-SH和熒光Cy5-PEG-SH的混合物對表面進行功能化AuNC@Fe3O4然后是熒光AuNC@Fe3O4與靶向分子葉酸結(jié)合形成熒光F-AuNC@Fe3O4用于靶向*癥細胞。

作為對照組，熒光AuNC@Fe3O4在沒有表面葉酸的情況下，也使用了葉酸，其物理性質(zhì)與熒光染料相似F-AuNC@Fe3O4.圖4a、b行顯示了A549細胞與熒光蛋白孵育后的共聚焦圖像F-AuNC@Fe3O4和AuNC@Fe3O4濃度為0.5的懸浮液?分別以mg/mL的濃度持續(xù)1小時。圖4a（i）所示的熒光圖像清楚地顯示了熒光的成功內(nèi)化F-AuNC@Fe3O4進入A549細胞。

與圖4b（i）相比，圖4a（i）中觀察到更亮的紅色發(fā)射（A549細胞與熒光燈孵育AuNC@Fe3O4)表示更多F-AuNC@Fe3O4可通過葉酸受體介導的內(nèi)吞作用內(nèi)化到A549細胞中F-AuNC@Fe3O4具有更強的*癥靶向識別能力。

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CY5-PEG-SH

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CY7.5-PEG-N3

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