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          您當(dāng)前所在位置：首頁(yè) > 功能化學(xué) > 發(fā)光材料 > 常規(guī)熒光染料 > 近紅外I區(qū)II區(qū) > 近紅外熒光染料IRDye 700 CREB Consensus Oligonucleotide
          
			
          
		
          
		
          
			
          
				
          
					
          
						
          
							
          
								
          近紅外熒光染料IRDye 700 CREB Consensus Oligonucleotide
          
								
          5′ -- AGA GAT TGC CTG ACG TCA GAG AGC TAG -- 3′  3′ -- TCT CTA ACG GAC TGC AGT CTC TCG ATC -- 5′  Underlined nucleotides are the binding site.
          
							
          
							
          
								
          
									
          
										
          
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                                                Q-0097387
                                                100mg
                                                
                                                    
          
                                                       1
                                                    
          
                                                          		
                                                
          詢價(jià)
          		
                                            
          
                                            
          
                                                Q-0097387
                                                250mg
                                                
                                                    
          
                                                        1
                                                    
          
                                                          		
                                                
          詢價(jià)
          		
                                            
          
                                            
          
                                                Q-0097387
                                                500mg
                                                
                                                    
          
                                                      1
                                                    
          
                                                          		
                                                
          詢價(jià)
          		
                                            
          
                                            
          
                                                Q-0097387
                                                1g
                                                
                                                    
          
                                                      1
                                                    
          
                                                          		
                                                
          詢價(jià)
          		
                                            
          
                                            
          
                                                Q-0097387
                                                5g
                                                
                                                    
          
                                                       1
                                                    
          
                                                          		
                                                
          詢價(jià)
          		
                                            
          

                                        									
          
								
          
								
          
									
          
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          IRDye 700 CREB Consensus Oligonucleotide
          

           
          

          5′ -- AGA GAT TGC CTG ACG TCA GAG AGC TAG -- 3′
          

          3′ -- TCT CTA ACG GAC TGC AGT CTC TCG ATC -- 5′
          

          Underlined nucleotides are the binding site. IRDye 700 oligonucleotides are supplied as 25 µL of 50 nM (or 50 fmol/µL) double-stranded DNA.
          

          CREB proteins are cellular transcription factors activated by phosphorylation of various kinases that bind to certain DNA sequences. For this reason, it is a common oligonucleotide used in EMSA to assess protein binding.
          

          After the addition of the DNA to the protein-buffer mix, reactions are incubated to allow protein binding to DNA. A typical incubation condition is 20-30 minutes at room temperature.
          

          Since IRDye infrared dyes are somewhat sensitive to light, it is best to keep binding reactions in the dark during incubation periods (e.g., put the tubes into a drawer or simply cover the rack containing tubes with aluminum foil). After the incubation period, 10X Orange Loading Dye (P/N 927-10100) is added to the binding reaction for electrophoresis.
          

           
          
                                    							
          
                            							
          
								
          
									
          
										參數(shù)信息
									
          
									
          
										外觀狀態(tài)：
										固體或粉末
									
          
									
          
										質(zhì)量指標(biāo)：
										95%+
									
          
									
          
										溶解條件：
										有機(jī)溶劑/水
									
          
									
          
										CAS號(hào)：
										N/A
									
          
									
          
										分子量：
										N/A
									
          
									
          
										儲(chǔ)存條件：
										-20℃避光保存
									
          
									
          
										儲(chǔ)存時(shí)間：
										1年
									
          
									
          
										運(yùn)輸條件：
										室溫2周
									
          
									
          
										生產(chǎn)廠家：
										西安pg電子官方生物科技有限公司
									
          
								
          
							
          
						
          
						
          
							
          
								
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          5′ -- CGC TTG ATG ACT CAG CCG GAA -- 3′  3′ -- GCG AAC TAC TGA GTC GGC CTT -- 5′  Underlined nucleotides are the binding site. IRDye 700 oligonucleotides are supplied as 25 ?L of 50 nM (or 50 fmol/?L) double-stranded DNA.
          
									
          
																		
          
										
										
          IRDye 680RD Streptavidin is supplied as a liquid in buffer containing 10 mM phosphate, 183 mM NaCl, 2.7 nM KCl, pH 7.4 with sodium azide 0.005% (w/v) as a preservative.