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          近紅外熒光染料IRDye 700 AP-1 Consensus Oligonucleotide

          5′ -- CGC TTG ATG ACT CAG CCG GAA -- 3′ 3′ -- GCG AAC TAC TGA GTC GGC CTT -- 5′ Underlined nucleotides are the binding site. IRDye 700 oligonucleotides are supplied as 25 ?L of 50 nM (or 50 fmol/?L) double-stranded DNA.

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          產(chǎn)品介紹

          IRDye 700 AP-1 Consensus Oligonucleotide

           

          5′ -- CGC TTG ATG ACT CAG CCG GAA -- 3′

          3′ -- GCG AAC TAC TGA GTC GGC CTT -- 5′

          Underlined nucleotides are the binding site. IRDye 700 oligonucleotides are supplied as 25 µL of 50 nM (or 50 fmol/µL) double-stranded DNA.

          AP-1 is a transcription factor that regulates gene expression and controls a number of cellular processes including differentiation, proliferation, and apoptosis. For this reason, it is a common oligo used in EMSA to assess protein binding.

          Since IRDye infrared dyes are somewhat sensitive to light, it is best to keep binding reactions in the dark during incubation periods (e.g., put the tubes into a drawer or simply cover the rack containing tubes with aluminum foil). After the incubation period, 10X Orange Loading Dye (P/N 927-10100) is added to the binding reaction for electrophoresis.

          參數(shù)信息
          外觀狀態(tài): 固體或粉末
          質(zhì)量指標(biāo): 95%+
          溶解條件: 有機(jī)溶劑/水
          CAS號(hào): N/A
          分子量: N/A
          儲(chǔ)存條件: -20℃避光保存
          儲(chǔ)存時(shí)間: 1年
          運(yùn)輸條件: 室溫2周
          生產(chǎn)廠家: 西安pg電子官方生物科技有限公司
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          IRDye 680RD Streptavidin is supplied as a liquid in buffer containing 10 mM phosphate, 183 mM NaCl, 2.7 nM KCl, pH 7.4 with sodium azide 0.005% (w/v) as a preservative.

          High Molecular Weight Optimized for labeling 1.0 mg of protein with molecular weight 45 - 200 kDa. Low Molecular Weight Optimized for labeling 1.0 mg of protein with molecular weight 14 - 45 kDa.