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Clearance kinetics of IRDye 800CW carboxylate. A single SCID mouse was injected with 1 nmol of non-reactive IRDye 800CW dye, and clearance monitored over time as indicated. Pseudocolor fluorescence is superimposed on a white light image. IRDye 800CW carboxylate dispersed rapidly and was completely cleared after 48 h. Originally published in Kovar, J., et al. (2007) Anal Biochem 367:1-12.

　　How is IRDye 800CW Carboxylate Used?

　　In vitro Cell-based Assays

　　As a control for cell-based assays that monitor binding of a dye-labeled agent.

　　Validation of optical agents for in vivo administration

　　Evaluation of binding specificity

　　In vivo Imaging

　　For evaluation of behavior and clearance of the dye itself.

　　Timing of dye clearance from the animal's body

　　Retention of dye in certain organs or sites (e.g., liver or kidneys)

　　Labeling Reaction Reference

　　As a standard to determine the amount of unreacted ("free") dye after IRDye 800CW conjugation and purification.

　　Residual unreacted dye may cause:

　　Artificially high values when dye/protein (D/P) ratio is calculated

　　Increased background fluorescence in biological assays

　　Dye Structure

　　Properties

　　Chemical Formula: C46H50N2Na4O15S4

　　Molecular Weight: 1091.10 g/mol

　　Exact Mass: 1090.17