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說明：生化研究，能使過氧化氫分解為水和氧

過氧化氫酶 來源于牛肝臟

Catalase from bovine liver

lyophilized powder, 2,000-5,000 units/mg protein

Frequently Asked Questions

Live Chat and Frequently Asked Questions are available for this Product.

Components

Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.

Application

Catalase acts as a natural antioxidant to study the roles of reactive oxygen species in gene expression and apoptosis. It has also been used to protect against oxidative damage to proteins, lipids, and nucleic acids. Industrially, catalses have been used to remove hydrogen peroxide added to milk and cheese, in textile bleaching, and to examine its positive effects on the viability of DNA-repair mutants of E. coli.

Biochem/physiol Actions

激活過氧化氫的分解，用于研究活性氧在基因表達(dá)和細(xì)胞凋亡中的作用的天然抗氧化劑。

This product doesn′t need any activators, but it is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogens bromide, 2-mercaptoethanol, dithiothreitol, dianisidnie and nitrate.

Caution

不建議冷凍溶液。

Preparation Note

由縮二脲測(cè)定的蛋白質(zhì)

Unit Definition

在 pH 7.0 和 25°C 下，一單位本品每分鐘可以分解 1.0μmol H2O2，同時(shí) H2O2 濃度從 10.3mM 降至 9.2mM，這是通過 A240 的降低速率來測(cè)量的。

Related Categories 1.11.1.6 Catalase, 1.x.x.x Oxidoreductases, Application Index, Diagnostic and Analytical Enzymes, Enzyme Class Index,

General Metabolic Enzymes, General Metabolic Enzymes A-H, 代謝組學(xué), 生化試劑, 細(xì)胞生物學(xué), 酶、**劑和底物

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Quality Level ELITE

form lyophilized powder

mol wt tetramer mol wt ~250 kDa

isoelectric point 5.4

storage temp. −20°C

Gene Information cow ... CAT(280743)