熒光染料及其激發(fā)和發(fā)射波長(zhǎng)峰值如下表：
熒光染料示例 | 激發(fā)光 |
Indo-1, Ca saturated | 331 nm |
Indo-1 Ca2+ | 346 nm |
Cascade Blue BSA pH 7.0 | 401 nm |
Cascade Blue | 398 nm |
LysoTracker Blue | 373 nm |
Alexa 405 | 401 nm |
LysoSensor Blue pH 5.0 | 374 nm |
LysoSensor Blue | 374 nm |
DyLight 405 | 399 nm |
DyLight 350 | 332 nm |
BFP (Blue Fluorescent Protein) | 380 nm |
Alexa 350 | 343 nm |
7-Amino-4-methylcoumarin pH 7.0 | 346 nm |
Amino Coumarin | 345 nm |
AMCA conjugate | 347 nm |
Coumarin | 360 nm |
7-Hydroxy-4-methylcoumarin | 360 nm |
7-Hydroxy-4-methylcoumarin pH 9.0 | 361 nm |
6,8-Difluoro-7-hydroxy-4-methylcoumarin pH 9.0 | 358 nm |
Hoechst 33342 | 352 nm |
Pacific Blue | 404 nm |
Hoechst 33258 | 352 nm |
Hoechst 33258-DNA | 352 nm |
Pacific Blue antibody conjugate pH 8.0 | 404 nm |
PO-PRO-1 | 434 nm |
PO-PRO-1-DNA | 435 nm |
POPO-1 | 433 nm |
POPO-1-DNA | 433 nm |
DAPI-DNA | 359 nm |
DAPI | 358 nm |
Marina Blue | 362 nm |
SYTOX Blue-DNA | 445 nm |
CFP (Cyan Fluorescent Protein) | 434 nm |
eCFP (Enhanced Cyan Fluorescent Protein) | 437 nm |
1-Anilinonaphthalene-8-sulfonic acid (1,8-ANS) | 375 nm |
Indo-1, Ca free | 346 nm |
1,8-ANS (1-Anilinonaphthalene-8-sulfonic acid) | 375 nm |
BO-PRO-1-DNA | 462 nm |
BOPRO-1 | 462 nm |
BOBO-1-DNA | 461 nm |
SYTO 45-DNA | 451 nm |
evoglow-Pp1 | 448 nm |
evoglow-Bs1 | 448 nm |
evoglow-Bs2 | 448 nm |
Auramine O | 431 nm |
DiO | 487 nm |
LysoSensor Green pH 5.0 | 447 nm |
Cy 2 | 489 nm |
LysoSensor Green | 447 nm |
Fura-2, high Ca | 336 nm |
Fura-2 Ca2+sup> | 336 nm |
SYTO 13-DNA | 488 nm |
YO-PRO-1-DNA | 491 nm |
YOYO-1-DNA | 491 nm |
eGFP (Enhanced Green Fluorescent Protein) | 488 nm |
LysoTracker Green | 503 nm |
GFP (S65T) | 489 nm |
BODIPY FL, MeOH | 502 nm |
Sapphire | 396 nm |
BODIPY FL conjugate | 503 nm |
MitoTracker Green | 490 nm |
MitoTracker Green FM, MeOH | 490 nm |
Fluorescein 0.1 M NaOH | 493 nm |
Calcein pH 9.0 | 494 nm |
Fluorescein pH 9.0 | 490 nm |
Calcein | 493 nm |
Fura-2, no Ca | 367 nm |
Fluo-4 | 494 nm |
FDA | 495 nm |
DTAF | 495 nm |
Fluorescein | 495 nm |
Fluorescein antibody conjugate pH 8.0 | 493 nm |
CFDA | 495 nm |
FITC | 495 nm |
Alexa Fluor 488 hydrazide-water | 493 nm |
DyLight 488 | 493 nm |
5-FAM pH 9.0 | 492 nm |
FITC antibody conjugate pH 8.0 | 495 nm |
Alexa 488 | 493 nm |
Rhodamine 110 | 497 nm |
Rhodamine 110 pH 7.0 | 497 nm |
Acridine Orange | 431 nm |
Alexa Fluor 488 antibody conjugate pH 8.0 | 499 nm |
BCECF pH 5.5 | 485 nm |
PicoGreendsDNA quantitation reagent | 502 nm |
SYBR Green I | 498 nm |
Rhodaminen Green pH 7.0 | 497 nm |
CyQUANT GR-DNA | 502 nm |
NeuroTrace 500/525, green fluorescent Nissl stain-RNA | 497 nm |
DansylCadaverine | 335 nm |
Rhodol Green antibody conjugate pH 8.0 | 499 nm |
Fluoro-Emerald | 495 nm |
Nissl | 497 nm |
Fluorescein dextran pH 8.0 | 501 nm |
Rhodamine Green | 497 nm |
5-(and-6)-Carboxy-2', 7'-dichlorofluorescein pH 9.0 | 504 nm |
DansylCadaverine, MeOH | 335 nm |
eYFP (Enhanced Yellow Fluorescent Protein) | 514 nm |
Oregon Green 488 | 498 nm |
Oregon Green 488 antibody conjugate pH 8.0 | 498 nm |
Fluo-3 | 506 nm |
BCECF pH 9.0 | 501 nm |
SBFI-Na+ | 336 nm |
Fluo-3 Ca2+ | 506 nm |
Rhodamine 123, MeOH | 507 nm |
FlAsH | 509 nm |
Calcium Green-1 Ca2+ | 506 nm |
Magnesium Green | 507 nm |
DM-NERF pH 4.0 | 493 nm |
Calcium Green | 506 nm |
Citrine | 515 nm |
LysoSensor Yellow pH 9.0 | 335 nm |
TO-PRO-1-DNA | 515 nm |
Magnesium Green Mg2+ | 507 nm |
Sodium Green Na+ | 507 nm |
TOTO-1-DNA | 514 nm |
Oregon Green 514 | 512 nm |
Oregon Green 514 antibody conjugate pH 8.0 | 513 nm |
NBD-X | 466 nm |
DM-NERF pH 7.0 | 509 nm |
NBD-X, MeOH | 467 nm |
CI-NERF pH 6.0 | 513 nm |
Alexa 430 | 431 nm |
Alexa Fluor 430 antibody conjugate pH 7.2 | 431 nm |
CI-NERF pH 2.5 | 504 nm |
Lucifer Yellow, CH | 428 nm |
LysoSensor Yellow pH 3.0 | 389 nm |
6-TET, SE pH 9.0 | 521 nm |
Eosin antibody conjugate pH 8.0 | 525 nm |
Eosin | 524 nm |
6-Carboxyrhodamine 6G pH 7.0 | 526 nm |
6-Carboxyrhodamine 6G, hydrochloride | 525 nm |
Bodipy R6G SE | 528 nm |
BODIPY R6G, MeOH | 528 nm |
6 JOE | 520 nm |
Cascade Yellow antibody conjugate pH 8.0 | 399 nm |
Cascade Yellow | 399 nm |
mBanana | 540 nm |
Alexa Fluor 532 antibody conjugate pH 7.2 | 528 nm |
Alexa 532 | 528 nm |
Erythrosin-5-isothiocyanate pH 9.0 | 533 nm |
6-HEX, SE pH 9.0 | 534 nm |
mOrange | 548 nm |
mHoneydew | 478 nm |
Cy 3 | 549 nm |
Rhodamine B | 543 nm |
DiI | 551 nm |
5-TAMRA-MeOH | 543 nm |
Alexa 555 | 553 nm |
Alexa Fluor 555 antibody conjugate pH 7.2 | 553 nm |
DyLight 549 | 555 nm |
BODIPY TMR-X, SE | 544 nm |
BODIPY TMR-X, MeOH | 544 nm |
PO-PRO-3-DNA | 539 nm |
PO-PRO-3 | 539 nm |
Rhodamine | 551 nm |
Bodipy TMR-X conjugate | 544 nm |
POPO-3 | 533 nm |
Alexa 546 | 562 nm |
BODIPY TMR-X antibody conjugate pH 7.2 | 544 nm |
Calcium Orange Ca2+ | 549 nm |
TRITC | 550 nm |
Calcium Orange | 549 nm |
Rhodaminephalloidin pH 7.0 | 558 nm |
MitoTracker Orange | 551 nm |
MitoTracker Orange, MeOH | 551 nm |
Phycoerythrin | 565 nm |
Magnesium Orange | 550 nm |
R-Phycoerythrin pH 7.5 | 565 nm |
5-TAMRA pH 7.0 | 553 nm |
5-TAMRA | 549 nm |
Rhod-2 | 552 nm |
FM 1-43 | 472 nm |
Rhod-2 Ca2+ | 553 nm |
Tetramethylrhodamine antibody conjugate pH 8.0 | 552 nm |
FM 1-43 lipid | 473 nm |
LOLO-1-DNA | 568 nm |
dTomato | 554 nm |
DsRed | 563 nm |
Dapoxyl (2-aminoethyl) sulfonamide | 372 nm |
Tetramethylrhodamine dextran pH 7.0 | 555 nm |
Fluor-Ruby | 554 nm |
Resorufin | 571 nm |
Resorufin pH 9.0 | 571 nm |
mTangerine | 568 nm |
LysoTracker Red | 578 nm |
Lissaminerhodamine | 572 nm |
Cy 3.5 | 578 nm |
Rhodamine Red-X antibody conjugate pH 8.0 | 573 nm |
Sulforhodamine 101, EtOH | 578 nm |
JC-1 pH 8.2 | 593 nm |
JC-1 | 592 nm |
mStrawberry | 575 nm |
MitoTracker Red | 578 nm |
MitoTracker Red, MeOH | 578 nm |
X-Rhod-1 Ca2+ | 580 nm |
Alexa Fluor 568 antibody conjugate pH 7.2 | 579 nm |
Alexa 568 | 576 nm |
5-ROX pH 7.0 | 578 nm |
5-ROX (5-Carboxy-X-rhodamine, triethylammonium salt) | 578 nm |
BO-PRO-3-DNA | 574 nm |
BOPRO-3 | 574 nm |
BOBO-3-DNA | 570 nm |
Ethidium Bromide | 524 nm |
ReAsH | 597 nm |
Calcium Crimson | 589 nm |
Calcium Crimson Ca2+ | 590 nm |
mRFP | 585 nm |
mCherry | 587 nm |
Texas Red-X antibody conjugate pH 7.2 | 596 nm |
HcRed | 590 nm |
DyLight 594 | 592 nm |
Ethidium homodimer-1-DNA | 528 nm |
Ethidiumhomodimer | 528 nm |
Propidium Iodide | 538 nm |
SYPRO Ruby | 467 nm |
Propidium Iodide-DNA | 538 nm |
Alexa 594 | 590 nm |
BODIPY TR-X, SE | 588 nm |
BODIPY TR-X, MeOH | 588 nm |
BODIPY TR-X phallacidin pH 7.0 | 590 nm |
Alexa Fluor 610 R-phycoerythrin streptavidin pH 7.2 | 567 nm |
YO-PRO-3-DNA | 613 nm |
Di-8 ANEPPS | 469 nm |
Di-8-ANEPPS-lipid | 469 nm |
YOYO-3-DNA | 612 nm |
Nile Red-lipid | 553 nm |
Nile Red | 559 nm |
DyLight 633 | 624 nm |
mPlum | 587 nm |
TO-PRO-3-DNA | 642 nm |
DDAO pH 9.0 | 648 nm |
Fura Red, high Ca | 434 nm |
Allophycocyanin pH 7.5 | 651 nm |
APC (allophycocyanin) | 650 nm |
Nile Blue, EtOH | 631 nm |
TOTO-3-DNA | 642 nm |
Cy 5 | 646 nm |
BODIPY 650/665-X, MeOH | 646 nm |
Alexa Fluor 647 R-phycoerythrin streptavidin pH 7.2 | 569 nm |
DyLight 649 | 652 nm |
Alexa Fluor 647 antibody conjugate pH 7.2 | 653 nm |
Alexa 647 | 653 nm |
Fura Red Ca2+ | 435 nm |
Atto 647 | 644 nm |
Fura Red, low Ca | 472 nm |
Carboxynaphthofluorescein pH 10.0 | 600 nm |
Alexa 660 | 664 nm |
Alexa Fluor 660 antibody conjugate pH 7.2 | 663 nm |
Cy 5.5 | 673 nm |
Alexa Fluor 680 antibody conjugate pH 7.2 | 679 nm |
Alexa 680 | 679 nm |
DyLight 680 | 678 nm |
Alexa Fluor 700 antibody conjugate pH 7.2 | 696 nm |
Alexa 700 | 696 nm |
FM 4-64, 2% CHAPS | 506 nm |
FM 4-64 | 508 nm |
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