文獻(xiàn)：線粒體、溶酶體和內(nèi)質(zhì)網(wǎng)：哪個(gè)是光療的最佳靶點(diǎn)？

鏈接：https://www.sciencedirect.com/science/article/abs/pii/S0168365922006320

作者：李艷紅 ，賈浩然 ，王洪 銀，華先武 ，鮑彥文 ，吳福 根

節(jié)選：

摘要

光動(dòng)力療法(PDT) 是一種強(qiáng)大的癌癥治療方式，其亞細(xì)胞作用位點(diǎn)的精確時(shí)空控制對(duì)其有效性至關(guān)重要。然而，準(zhǔn)確比較不同細(xì)胞器靶向 PDT 方法的療效具有挑戰(zhàn)性，因?yàn)楹茈y找到一個(gè)單一的系統(tǒng)能夠?qū)崿F(xiàn)對(duì)不同細(xì)胞器的單獨(dú)靶向性、可分離的時(shí)間窗和相似的結(jié)合量。在此，我們將二氫卟酚 e6 (Ce6) 與 1,2-二硬脂酰-sn-甘油-3-磷酸乙醇胺-N- [氨基(聚乙二醇)-5000]（銨鹽）（DSPE-PEG 5000 -NH 2）偶聯(lián)得到 DSPE-PEG-Ce6，其在細(xì)胞內(nèi)化后可以從線粒體遷移到溶酶體，最終到內(nèi)質(zhì)網(wǎng) (ER)。得益于DSPE-PEG-Ce6亞細(xì)胞分布的動(dòng)態(tài)性以及其與細(xì)胞器結(jié)合量的可調(diào)性，我們準(zhǔn)確地確定了該分子的光動(dòng)力治療（PDT）療效順序，即線粒體 > 內(nèi)質(zhì)網(wǎng) > 溶酶體。本研究提出了一個(gè)理想的模型系統(tǒng)，可用于準(zhǔn)確評(píng)估特定細(xì)胞器靶向的光動(dòng)力治療（PDT）療效，并有望促進(jìn)未來(lái)有效光動(dòng)力治療策略的發(fā)展。

Abstract

Photodynamic therapy (PDT) is a robust cancer treatment modality, and the precise spatiotemporal control of its subcellular action site is crucial for its effectiveness. However, accurate comparison of the efficacy of different organelle-targeted PDT approaches is challenging since it is difficult to find a single system that can achieve separate targeting of different organelles with separable time windows and similar binding amounts. Herein, we conjugated chlorin e6 (Ce6) with 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-5000] (ammonium salt) (DSPE-PEG5000-NH2) to afford DSPE-PEG-Ce6, which could migrate from mitochondrion to lysosome and ultimately to endoplasmic reticulum (ER) after cellular internalization. Benefiting from the dynamic subcellular distribution of DSPE-PEG-Ce6 with tunable organelle-binding amounts, we accurately determined the PDT efficacy order of the molecule, i.e., mitochondrion > ER > lysosome. This work proposes an ideal model system for accurately evaluating the specific organelle-targeted PDT efficacy and may promote the future development of effective PDT strategies.

西安pg電子官方生物提供相關(guān)產(chǎn)品：

SP94-PEG-DSPE

T7(HAIYPRH)-PEG-DSPE

Octreotide-PEG-DSPE

YIGSR-PEG-DSPE

APRPG-PEG-DSPE

NGR-PEG-DSPE

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