TP-FLIM雙光子熒光壽命成像光學(xué)造影劑

雙光子熒光壽命成像（Two-photon fluorescence lifetime imaging, TP-FLIM）技術(shù)集合了雙光子熒光成像和熒光壽命成像的優(yōu)勢(shì)，能夠?qū)ι�物組織進(jìn)行高分辨三維成像的同時(shí)提供生物組織的生化特性信息，在**診療方面具有巨大的臨床應(yīng)用前景。為了獲得較佳的雙光子熒光壽命成像效果，光學(xué)造影劑除了需要具有較強(qiáng)的雙光子吸收，用于降低激光的能量，避免高能量激光對(duì)組織的損傷；還需要具有穩(wěn)定且較長(zhǎng)的熒光壽命，用于去除生物自熒光對(duì)雙光子熒光壽命成像的干擾。然而，目前還鮮有材料能夠同時(shí)滿足上述要求。因此，迫切需要設(shè)計(jì)合成一種合適光學(xué)造影劑，用于推動(dòng)雙光子熒光壽命成像技術(shù)在生物診療領(lǐng)域的發(fā)展。

一種基于熱激活延遲熒光（Thermally activated delayed ?uorescence, TADF）的有機(jī)光學(xué)造影劑，可用于雙光子活體熒光壽命成像。在該工作中，研究者充分利用熱激活延遲熒光材料通常具有較長(zhǎng)延遲熒光（Delayed fluorescence）壽命這一優(yōu)點(diǎn)，將具有聚集誘導(dǎo)熒光增強(qiáng)的有機(jī)熱激活延遲熒光材料包覆于兩親性共聚物內(nèi)，成功制備了水溶性的有機(jī)納米顆粒。光學(xué)研究表明兩親性共聚物的包覆較大化了熱激活延遲熒光材料的聚集態(tài)，大大增加了材料的熒光壽命，獲得了可媲美磷光材料的超長(zhǎng)發(fā)光壽命（4.2 μs）。更為重要的是，不像傳統(tǒng)磷光發(fā)光強(qiáng)度和壽命容易被氧氣猝滅，該材料對(duì)氧氣表現(xiàn)出明顯的惰性，即便在富氧區(qū)域仍然表現(xiàn)出穩(wěn)定的熒光壽命，非常有利于獲得高信噪比的熒光壽命成像。同時(shí)，該熱激活延遲熒光材料由于具有Donor-Acceptor（D-A）結(jié)構(gòu)，表現(xiàn)出良好的雙光子吸收截面，輕易實(shí)現(xiàn)了活體雙光子熒光壽命成像。

該研究為雙光子熒光壽命成像技術(shù)提供了一種良好的光學(xué)造影劑，對(duì)推動(dòng)雙光子熒光壽命成像技術(shù)在臨床**診療中的發(fā)展具有重要意義。

活體熒光造影劑    

Sulfo-CY7(Cyanine7)NHSester    

Sulfo-Cyanine7.5NHSester,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

sulfo-CY7(Cyanine7)maleimide    

sulfo-Cyanine7.5maleimide,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

Sulfo-CY7(Cyanine7)dicarboxylicacid    

Sulfo-Cyanine7.5dicarboxylicacid,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

Sulfo-CY7(Cyanine7)carboxylicacid    

Sulfo-Cyanine7.5carboxylicacid,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

Sulfo-CY7(Cyanine7)azide    

Sulfo-Cyanine7.5azide,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

Sulfo-CY7(Cyanine7)alkyne    

Sulfo-Cyanine7.5alkyne,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

Sulfo-CY7(Cyanine7)tetrazine    

Sulfo-Cyanine7tetrazine,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

Sulfo-CY7(Cyanine7)NHSester    

Sulfo-Cyanine7NHSester,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

Sulfo-CY7(Cyanine7)maleimide    

Sulfo-Cyanine7maleimide,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

sulfo-CY7(Cyanine7)dicarboxylicacid    

sulfo-Cyanine7dicarboxylicacid,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

Sulfo-CY7(Cyanine7)carboxylicacid    

Sulfo-Cyanine7carboxylicacid,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

Sulfo-CY7(Cyanine7)bis-NHSester    

Sulfo-Cyanine7bis-NHSester,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

Sulfo-CY7(Cyanine7)azide    

Sulfo-Cyanine7azide,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

Sulfo-CY7(Cyanine7)amine    

Sulfo-Cyanine7amine,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

Sulfo-CY7(Cyanine7)alkyne    

Sulfo-Cyanine7alkyne,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)tetrazine    

Cyanine7.5tetrazine,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)NHSester    

Cyanine7.5NHSester,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)maleimide    

Cyanine7.5maleimide,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)hydrazide    

Cyanine7.5hydrazide,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)carboxylicacid    

Cyanine7.5carboxylicacid,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)azide    

Cyanine7.5azide,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)azide,10mM/DMSO    

Cyanine7.5azide,10mM/DMSO,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)amine    

Cyanine7.5amine,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)alkyne    

Cyanine7.5alkyne,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)tetrazine    

Cyanine7tetrazine,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)NHSester    

Cyanine7NHSester,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)maleimide    

Cyanine7maleimide,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)hydrazide    

Cyanine7hydrazide,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)dicarboxylicacid    

Cyanine7dicarboxylicacid,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)DBCO    

Cyanine7DBCO,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)carboxylicacid    

Cyanine7carboxylicacid,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)azide    

Cyanine7azide,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)azide,10mM/DMSO    

Cyanine7azide,10mM/DMSO,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)amine    

Cyanine7amine,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

CY7(Cyanine7)alkyne    

Cyanine7alkyne,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics    

BDP650/665XNHSester    

BDP650/665XNHSester,Borondipyrromethenedyeforfluorescein(516nm)channel-verybrightandphotostablefluorophore.    

BDP650/665maleimide    

BDP650/665maleimide,Borondipyrromethenedyeforfluorescein(515nm)channel-verybrightandphotostablefluorophore.    

BDP650/665azide    

BDP650/665azide,Borondipyrromethenedyeforfluorescein(514nm)channel-verybrightandphotostablefluorophore.    

BDP650/665alkyne    

BDP650/665alkyne,Borondipyrromethenedyeforfluorescein(513nm)channel-verybrightandphotostablefluorophore.    

BDP630/650XNHSester    

BDP630/650XNHSester,Borondipyrromethenedyeforfluorescein(512nm)channel-verybrightandphotostablefluorophore.    

BDP630/650tetrazine    

BDP630/650tetrazine,Borondipyrromethenedyeforfluorescein(511nm)channel-verybrightandphotostablefluorophore.    

BDP630/650maleimide    

BDP630/650maleimide,Borondipyrromethenedyeforfluorescein(510nm)channel-verybrightandphotostablefluorophore.    

BDP630/650hydrazide    

BDP630/650hydrazide,Borondipyrromethenedyeforfluorescein(509nm)channel-verybrightandphotostablefluorophore.    

BDP630/650carboxylicacid    

BDP630/650carboxylicacid,Borondipyrromethenedyeforfluorescein(508nm)channel-verybrightandphotostablefluorophore.    

BDP630/650azide    

BDP630/650azide,Borondipyrromethenedyeforfluorescein(507nm)channel-verybrightandphotostablefluorophore.    

BDP630/650amine    

BDP630/650amine,Borondipyrromethenedyeforfluorescein(506nm)channel-verybrightandphotostablefluorophore.    

BDP630/650alkyne    

BDP630/650alkyne,Borondipyrromethenedyeforfluorescein(505nm)channel-verybrightandphotostablefluorophore.    

上述產(chǎn)品pg電子官方生物均可供應(yīng)，僅用于科研！

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