名稱：pGBKT7-53 Vector 酵母雙雜交對(duì)照載體

其他名稱：pGBKT7-53 Control vector

規(guī)格：2μl

運(yùn)輸溫度：干冰運(yùn)輸

保存溫度：-20℃保存

**期：1年

應(yīng)用：酵母雙雜交

基本信息載體類型:酵母雙雜交系統(tǒng)裁體

載體大小:8.3kb

原核抗性:卡那霉素(Kanamycin, Kan )

克隆菌株:DH5a

載體標(biāo)簽c-Myc

篩選標(biāo)記:TRP1

啟動(dòng)子:ADH1

表達(dá)宿主:酵母細(xì)胞

5'測(cè)序引物:T7:TAATACGACTCACTATAGGG

3測(cè)序引物:根據(jù)序列設(shè)計(jì)引物

質(zhì)粒圖譜：

描述∶

pGBKT7-53是陽(yáng)性對(duì)照質(zhì)粒，能編碼小鼠p53蛋白(a.a.72-390)和GAL4 DNA-BD(a.1-147)的融合蛋白。小鼠p53 cDNA克隆進(jìn)入pGBKT7的EcoR和BanH1位點(diǎn)之間。p53 cDNA來(lái)源于Lwabuchi et al. ( 1993 )年文獻(xiàn)提到的質(zhì)粒（由Clontech改造所得)。

保存與運(yùn)輸方法：

保存:-20°C保存，至少1年**。運(yùn)輸:冰袋運(yùn)輸。

注意事項(xiàng)：

我司提供的載體/質(zhì)粒/菌株都經(jīng)過(guò)嚴(yán)格的質(zhì)量控制。請(qǐng)收到貨后**行轉(zhuǎn)化，再挑取單克隆擴(kuò)增。產(chǎn)品使用中若遇到質(zhì)量問(wèn)題，請(qǐng)和我司及時(shí)溝通。為了您的安全和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。

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yyp2021.2.4